Aim: Use single-cell RNAseq technology to determine the cellular response in all cell types in the aging brain.
The molecular and cellular changes of the aging brain have been actively studied for many years by various groups. Transcriptomic studies have identified numerous changes that are associated with aged brains in model organisms and in human postmortem tissue. Many of these changes are immune related and it is unclear whether this is due to glial activation or small but important peripheral immune cell infiltration into the brain. The aim of this study is to use single-cell RNAseq technology to determine the cellular response in all cell types in the aging brain. We will utilize the 10XGenomics Chromium platform to profile cortex, hippocampus, and peripheral blood mononuclear cells (PBMCs) with ~5000 cells/region and a read depth of >200K reads/cell. This will provide a rich dataset that we will prepare for publication to disseminate to the scientific community. More importantly, these data will lead to new hypotheses of cellular and molecular changes that we plan to follow with future functional studies.