Animal and Phenotyping Core

Animal and Phenotyping Core

Animal and Phenotyping Core Major Goals

  • Conduct lifespan and healthspan studies in DO and CC mice
  • Implement a caloric restriction (CR) study in DO mice
  • Develop and provide affordable phenotyping
  • Collect and store tissues
  • Coordinate with the Translational Core to obtain knockout (KO) models for lifespan studies.
  • Coordinate with the Data and Statistical Core to analyze data and streamline dissemination efforts to the aging community.

Origins of Collaborative Cross (CC) Mice

Recombinant inbred lines generated from an 8-way cross

 

Origins of Diversity Outcross (DO) Mice

Diversity Outbred (DO) mice are derived from 147 distinct, partially inbred Collaborative Cross (CC) lines and are maintained as an outbred stock.  Hence levels of heterozygosity are similar to what is seen in humans and the effects of lethal recessives are removed.

Origins of Diversity Outbred (DO) mice II

The DO captures 90% of the genetic diversity among laboratory inbred strains. Panels of DO mice can be used for high resolution genetic mapping.

Each DO mouse is unique, capturing a unique combinations of alleles from the 8 founder strains.

Phenotyping HIghlights

Evolutionary Theory of Aging

Lifespan correlates with sexual maturation (age of vaginal patency) in inbred mouse strains.

  • Yuan R, Meng Q, Nautiyal J, Flurkey K, Tsaih SW, Krier R, Parker MG, Harrison DE, Paigen B
    Genetic coregulation of age of female sexual maturation and lifespan through circulating IGF1 among inbred mouse strains
    Proc Natl Acad Sci U S A. 2012 May 22;109(21):8224-9. doi: 10.1073/pnas.1121113109

    [ Epub 2012 May 7 ]

Phenotyping Highlights

Development of a novel assay for bladder function and urinary incontinence in mice

A spontaneous voiding assay that is simple, reproducible, quantitative, and noninvasive developed in conjunction with collaborators at BIDMC/HMS

FIGURE:

Individual mice exhibit reproducible patterns of urination that are strain unique.
Top: photographs showing filters collected over 4 days from two individual mice
of two different inbred strains.Bottom: quantitative data extracted from four mice
of each strain assayed on 4 consecutive days in the afternoon. All three
parameters (urine spot number, percentarea in the primary void, and total urine spot
area) were highly statistically different by Student's t-test (P values are indicated).

  • Yu W, Ackert-Bicknell C, Larigakis JD, MacIver B, Steers WD, Churchill GA, Hill WG, Zeidel ML
    Spontaneous voiding by mice reveals strain-specific lower urinary tract function to be a quantitative genetic trait
    Am J Physiol Renal Physiol. 2014 Jun 1;306(11):F1296-307. doi: 10.1152/ajprenal.00074.2014

    [ Epub 2014 Apr 9 ]